學術資源整合系統-相關推薦

 
作者Jeng, M.-L.;M.-Y. Chen;L.-W. Wu
出版日期2021
著作名稱Two complete mitochondrial genomes of Papilio butterflies obtained from historical specimens (Lepidoptera: Papilionidae).
刊名Mitochondrial DNA Part B Resources
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4
頁數1341-1343.
被收錄索引SCI
主題動物
關鍵字mitogenomic phylogeny, Pterourus, Chilasa, Heraclides, Meta-genomics
摘要Museum specimens are collected for education, exhibition, and various multiple scientific purposes. However, millions of specimens remain in their collection boxes for years without being analyzed. Historical specimens have been known to contain low-quality DNA; hence, it is difficult to utilize their sequence information in phylogenetic studies. However, recent advances in high-throughput sequencing (HTS) make these collections amenable to phylogenomic studies. In this study, two historical specimens (Papilio xuthus Linnaeus, 1767, and Papilio thoas Linnaeus, 1771) were sampled and DNA extracted for HTS via the Miseq platform. Two complete mitogenomes were assembled, even though the DNA quality of those specimens was highly fragmented, below 250 bp in length. The 37 genes of 60 mitogenomes were aligned and used for inferring the phylogenetic relationships of Papilioninae. These two newly sequenced mitogenomes are correctly grouped in the genus Papilio, and this result indicates that historical specimens show great potential for phylogenetic studies with HTS technology.
全文Full Text
DOIhttps://doi.org/10.1080/23802359.2021.1909443
系統號NO000006014

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