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作者Chao, W.C.;Y.C. Liu;M.T. Jiang;S.S. Wu;C.L. Fang;J.F. Ho;C.L. Huang
出版日期2021
已接受202012
接受證明Full Text
著作名稱Genetic diversity, population genetic structure and conservation strategies for Pleione formosana (Orchideace)
刊名Taiwania
66
1
頁數20-30
被收錄索引SCI
主題植物
關鍵字Pleione, genotyping by sequencing (GBS); next-generation sequencing; Orchidaceae; population genomics
摘要A population genomic approach was employed to investigate the diversity within species to create a more robust, lineage-specific conservation strategy for an endangered orchid. Pleione formosana is a species native to southeastern China and Taiwan, where it is distributed at an altitude range of 1,200 - 2,500 m the foggy mountain area and grows in mosses on half-shaded rocks or tree trunks. To identify whether the level of genetic diversity in the species, we used genotyping by sequencing (GBS) to analysis the sub-populations of the species. Fifty-eight individuals of P. formosana were sampled from a total of nine populations within continental island Taiwan and three populations of an outgroup in China. Treatment of all samples involved five major steps: sample preparation, library assembly, sequencing, SNP calling and diversity analysis. GBS markers confirmed the China outgroup as distinct, and provided resolution of two clusters of population genetic structure in Taiwan. Outliers provide higher genetic differentiation, and some GBS tags associated with climatic factor were found. Genomic diversity identified among the three clusters suggests that conservation of this species will be best served by considering them as three evolutionary significant units (ESUs). This approach will maximize evolutionary potential among all species during increased isolation and environmental change. According to the genetic consequences, restoration strategies should be carried out in all populations to preserve genetic diversity and evolutionary potential for different environmental factors.
系統號NO000005873

Aug 20 2021 11:12:17
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