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作者Zhang, Shichang;Piorkowski, Dakota;Lin, Wan-Rou;Lee, Yi-Ru;Liao, Chen-Pan;Wang, Pi-Han;Tso, I-Min
出版日期201910
著作名稱Nitrogen inaccessibility protects spider silk from bacterial growth
刊名Journal of Experimental Biology
222
頁數jeb214981
被收錄索引SCI
主題動物
關鍵字Bacteriostatic characteristics, Cyrtophora moluccensis, Hippasa holmerae, Nephila pilipes, Spider silk
摘要Spider silks are protein-based fibers that are incorporated into webs with the unique combination of high mechanical toughness and resistance to microbial degradation. While spiders are undoubtedly exposed to saprophytic microorganisms in their native habitats, such as the forest understory and bush, their silks have rarely been observed to decompose in either field or laboratory studies. We performed cross-streaking assays using silk from three spider species and four bacterial strains and found no inhibition zones, indicating the absence of antibacterial properties. We also cultured all bacteria directly upon silk in Luria–Bertani (LB) broth (full nutrients), phosphate-buffered saline (PBS; no nutrients) and nitrogen-free glucose broth (NFG; full nutrients, no nitrogen), and found that bacteria grew readily on silk in LB broth but not in PBS or NFG buffer. Our results indicate that spider silk's resistance to bacterial degradation is likely due to bacteriostatic rather than antibacterial mechanisms when nitrogen is inaccessible.
資料連結Full Text
DOI10.1242/jeb.214981
系統號NO000005408

Aug 20 2021 11:12:17
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