學術資源整合系統-相關推薦
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| 作者 | I-Ling Lai;Chih-Wan Lin;Tsai-Yu Chen;Wei-Hsin Hu |
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| 出版日期 | 201812 |
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| 已接受 | 20180927 |
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| 著作名稱 | Micropropagation Shortens the Time to Blooming of Begonia montaniformis x Begonia ningmingensis var. bella F1 Progeny
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| 刊名 | HortScience |
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| 卷 | 53 |
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| 期 | 12 |
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| 被收錄索引 | SCI |
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| 主題 | 植物 |
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| 關鍵字 | plant growth regulator, light quality, shoot organogenesis, Begonia |
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| 摘要 | Begonia montaniformis 3 Begonia ningmingensis var. bella hybrids have high
ornamental potential. Hence, the aim of this study was to determine the optimal
conditions for the micropropagation of a Begonia montaniformis 3 Begonia ningmingensis var. bella F1 progeny by using various concentrations of plant growth regulators(PGRs) and varying light spectra in half-strength Murashige and Skoog (1/2 MS)medium. The results showed that the explant regeneration was optimal when the laminawas incubated in a medium supplemented with 2.0 mM N6-benzylaminopurine and 0.8 mM a-naphthaleneacetic acid (NAA). Under such conditions, 98% of the explants regenerated adventitious shoots after 8 weeks, and 41 buds were produced per explant on average. The mean shoot length was 9.6 mm, and on average, 4.5 shoots per explant were more than 2 mm long. Subsequently, the induced adventitious shoots were transferred
into rooting medium consisting of 1/2 MS and various NAA concentrations. After 4
weeks, the shoots subcultured in this medium showed ’93% root induction and an
average of 3.5 adventitious roots per explant. Furthermore, the applied light spectrum significantly influenced shoot regeneration, and optimal results were achieved under an equal distribution of blue, red, and infrared light. The histological sections of shoots regenerated from direct organogenesis were observed through scanning electron microscopy (SEM). Afterward, the rooting adventitious shoots were subcultured in PGR-free medium for 8 weeks. The seedlings were successfully acclimated 4 weeks after being transferred to soil and bloomed after 11 months in a greenhouse. Thus, the PGR composition in micropropagation efficiently shortened the time to blooming from 25 to 16
months. |
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| 系統號 | NO000004877 |
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