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作者Hu, W.H;Liu, S.F.; Liaw, S.I.
出版日期2015
著作名稱Long-term preconditioning of plantlets: a practical method for enhancing survival of pineapple (Ananas comosus (L.) Merr.) shoot tips cryopreserved using vitrification.
刊名CryoLetters
36
4
頁數226-236
被收錄索引SCI
摘要BACKGROUND: The purpose of this study was to develop an efficient cryopreservation protocol for pineapple (Ananas comosus (L.) Merr.) shoot tips. OBJECTIVE: The optimal state of pineapple plantlets was investigated by using sucrose preconditioning to enhance survival after cryostorage. METHODS: To achieve a suitable state of plantlets before cryopreservation, 0.2 M to 0.4 M sucrose concentrations combined with short- (0–7 days), medium- (15–30 days), and long-term (75–150 days) preconditioning periods were compared. RESULTS AND CONCLUSION: The highest survival (100%) was achieved using the following procedure: intact plantlets underwent long-term preconditioning with 0.2 M sucrose for 135 days, dissected shoot tips were treated with a loading solution containing 2.0 M glycerol 0.4 M sucrose for 60 min at 25°C and the shoot tips were dehydrated in PVS2 for 2 h at 0°C before being plunged in liquid nitrogen. Rewarming was conducted in a water-bath for 30 s at 40°C and PVS2 was replaced with a 1.2 M sucrose solution for 30 min at 25°C. The shoot tips were transferred on semisolid medium and left in the dark for 1 week, then in dim light for 3 weeks.
系統號NO000003906

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