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作者Pei, N.; D.L. Erickson; B. Chen X. Ge, X. Mi, N.G. Swenson, J.L. Zhang, F.A. Jones, C.L. Huang, W. Ye, Z. Hao, C.F. Hsieh, S. Lum, N.A. Bourg, J.D. Parker, J.K. Zimmerman, W.J. McShea, et al; X. Ge, X. Mi, N.G. Swenson, J.L. Zhang, F.A. Jones, C.L. Huang, W. Ye, Z. Hao, C.F. Hsieh, S. Lum, N.A. Bourg, J.D. Parker, J.K. Zimmerman, W.J. McShea, et al.
出版日期2015
著作名稱Closely-related taxa influence woody species discrimination via DNA barcoding: evidence from global forest dynamics plots
刊名Scientific Reports
5
頁數15127
摘要To determine how well DNA barcodes from the chloroplast region perform in forest dynamics plots (FDPs) from global CTFS-ForestGEO network, we analyzed DNA barcoding sequences of 1277 plant species from a wide phylogenetic range (3 FDPs in tropics, 5 in subtropics and 5 in temperate zone) and compared the rates of species discrimination (RSD). We quantified RSD by two DNA barcode combinations (rbcL matK and rbcL matK trnH-psbA) using a monophyly-based method (GARLI). We defined two indexes of closely-related taxa (Gm/Gt and S/G ratios) and correlated these ratios with RSD. The combination of rbcL matK averagely discriminated 88.65%, 83.84% and 72.51% at the local, regional and global scales, respectively. An additional locus trnH-psbA increased RSD by 2.87%, 1.49% and 3.58% correspondingly. RSD varied along a latitudinal gradient and were negatively correlated with ratios of closely-related taxa. Successes of species discrimination generally depend
on scales in global FDPs. We suggested that the combination of rbcL matK trnH-psbA is currently applicable for DNA barcoding-based phylogenetic studies on forest communities.
系統號NO000003901

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