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作者Lin, J. L.;Gu, S. H.
出版日期2007
著作名稱In vitro and in vivo stimulation of extracellular signal-regulated kinase (ERK) by prothoracicotropic hormone in prothoracic gland cells and its developmental regulation in the silkworm, Bombyx mori.
刊名Journal of Insect Physiology
53
6
頁數622-631
被收錄索引SCI
關鍵字Bombyx mori; ERK; PTTH
摘要In this study, we investigated activation of the extracellular signal-regulated kinase (ERK) by the prothoracicotropic hormone (PTTH) in prothoracic gland cells of the silkworm, Bombyx mori. The results showed that the PTTH stimulated ERK phosphorylation as this depends on time and dose and ecdysteroidogenic activity. The ERK phosphorylation inhibitors, PD 98059 and U0126, blocked both basal and PTTH-stimulated ERK phosphorylation and ecdysteroidogenesis. In addition, activation of glandular ERK phosphorylation by the PTTH appeared to be developmentally regulated with the refractoriness of gland cells to the PTTH occurring during the latter stages of both the fourth and last larval instars. Moreover, in vitro activation of ERK phosphorylation of prothoracic glands by the PTTH was also verified by in vivo experiments: injection of the PTTH into day 6 last instar larvae greatly increased the activity of glandular ERK phosphorylation and ecdysteroidogenesis. These results suggest that development-specific changes in ERK phosphorylation may play a role in PTTH stimulation of ecdysteroidogenesis.
DOI10.1016/j.jinsphys.2007.03.004
系統號NO000000216

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