學術資源整合系統-相關推薦
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| 作者 | Gu Shi-Hong |
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| 出版日期 | 202605 |
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| 著作名稱 | Temporal transcriptional responses of insulin-associated metabolic genes during chilling-induced embryonic diapause termination in Bombyx mori
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| 刊名 | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
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| 卷 | 315 |
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| 頁數 | 111988 |
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| 被收錄索引 | SCI;SCI |
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| 主題 | 動物;動物 |
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| 關鍵字 | Chilling; Diapause termination; Embryonic diapause; Glycogen phosphorylase; Glycogen synthase; Insulin signaling; Mef2; Metabolism; Mitf. |
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| 摘要 | Understanding how low-temperature signals are integrated into transcriptional regulatory networks is essential for elucidating the mechanisms underlying chilling-induced termination of embryonic diapause. In Bombyx mori, several signaling pathways, including insulin signaling, have been implicated in this process; however, the transcriptional behavior of metabolism-related genes associated with insulin signaling during prolonged chilling remains incompletely characterized. In this study, I examined the temporal expression patterns of a targeted set of insulin-associated genes, including components of insulin signaling (insulin receptor [InR], forkhead box protein O [FOXO]), glycogen and glucose metabolism (glycogen synthase [Glys], glycogen phosphorylase [Glyp], glucose-6-phosphate dehydrogenase [G6PD]), and the transcription factors (myocyte enhancer factor 2 [Mef2] and microphthalmia-associated transcription factor [Mitf]), during chilling-induced diapause termination. By initiating chilling 2 or 15 days after oviposition, diapause eggs with weaker or stronger diapause intensity, respectively, were obtained. Their temporal responses to chilling were compared with those kept at 25 °C. Upon transfer to 5 °C at 2 days post-oviposition, InR transcripts increased 15 days after transfer and then maintained a relatively higher level thereafter. Increased FOXO transcripts were also detected between 30 and 60 days after transfer. While other genes showed gene-specific expression patterns during chilling. Following transfer to 5 °C at 15 days post-oviposition, all examined genes showed increased expression compared with those maintained at 25 °C. Returning eggs that had been chilled for 30 days to 25 °C resulted in reduced expression of FOXO, Glys, Glyp, G6PD, Mef2, and Mitf, indicating that a 30-day chilling period is not sufficient to sustain elevated transcription of these genes. These results provide a temporal framework describing chilling-dependent transcriptional regulation of selected insulin-associated metabolic genes during embryonic diapause termination in B. mori. While the findings do not directly assess metabolic activity, they identify candidate regulatory components whose functional roles in carbohydrate metabolism and diapause termination warrant further investigation. |
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| 資料連結 | ![Full Text]() |
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| 計畫主持人 | 顧世紅 |
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| 計畫編號 | NSTC 114-2313-B-178-001 |
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| 計畫年度 | 2025 |
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| 計畫委託單位 | 國家科學及技術委員會 |
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| 計畫名稱 | 昆蟲胰島素的新功能：前胸腺自體分泌因子的探索(二) |
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| 系統號 | NO000007729 |
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