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| 作者 | Hu, Wei-Hsin;Yang, Kai-Jr;Lai, I-Ling;Liaw, Song-Iuan |
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| 出版日期 | 202409 |
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| 著作名稱 | Cryopreservation of the vulnerable medicinal plant Nothapodytes nimmoniana (J. Graham) Mebb.’s shoot tips using the vitrification technique: effects of the vitrification procedure and recovery media
=藥用植物青脆枝玻璃化法超低溫保存之研 究：玻璃化程序及培養基對存活率的影響
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| 刊名 | Taiwan Journal of Biodiversity |
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| 卷 | 26 |
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| 期 | 3 |
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| 頁數 | 54-78 |
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| 主題 | 植物;植物 |
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| 關鍵字 | Nothapodytes nimmoniana, cryopreserved, sucrose pretreatment, PVS2 |
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| 摘要 | Nothapodytes nimmoniana (J. Graham) Mebb. is a highly valuable anticancer
medicinal plant and a near-threatened species on the Red List of Taiwan Plants. This
study aims to study the influence of the vitrification technique’s treatment procedures and recovery media on the survival of in vitro shoot tips in cryopreservation. To prepare the explants for a suitable state before cryopreservation, the pretreatment involved comparing sucrose concentrations ranging from 0.3 M to 0.7 M and durations ranging from one to seven days following the 0.7 M concentration. Additionally, to ensure reliable cryopreserved results, we tested the cryoprotectant (PVS2) dehydration period and the recovery medium after post-thawing. The results of sucrose pretreatment
after non-cryopreservation showed that the highest survival rates, 72.6% and 73.3%,
were achieved either with a 0.7 M sucrose concentration or after a three-day sucrose
pretreatment. These results are based on the following procedure: intact plantlets
underwent different pretreatment processes, in which dissected shoot tips were treated
with a loading solution containing 2.0 M glycerol and 0.4 M sucrose for 60 minutes at
25°C, and the shoot tips were subsequently dehydrated in PVS2 for 90 minutes at 0°C
before being plunged into liquid nitrogen. The optimum cryoprotectant dehydration for
90 minutes resulted in a 68.8% survival rate after cryopreservation. Rewarming was
conducted in a water bath for 30 seconds at 40°C, and PVS2 was replaced with a 1.2
M sucrose solution for 30 minutes at 25°C. Shoot tips transferred to WPM medium
supplemented with 1.0 mg⋅L–1 BA and 0.1 mg⋅L–1 IBA achieved a 73.3% survival rate
after four weeks of recovery. The procedure provided another high survival rate of in
vitro preservation of N. nimmoniana. |
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| 全文 | ![Full Text]() |
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| 資料連結 | ![Full Text]() |
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| 系統號 | NO000007054 |
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