學術資源整合系統-相關推薦
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| 作者 | Shi-Hong Gu;Pei‐Ling Lin;Chia-Hao Chang |
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| 出版日期 | 2023 |
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| 著作名稱 | Expressions of sugar transporter genes during Bombyx mori embryonic development
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| 刊名 | Journal of Experimental Zoology Part A: Ecological Genetics and Physiology |
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| 卷 | 339 |
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| 期 | 8 |
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| 頁數 | 788-798 |
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| 被收錄索引 | SCI;SCI |
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| 主題 | 動物;植物 |
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| 關鍵字 | Bombyx, insulin signaling, sugar transporters, PI3K, embryonic diapause, ecdysone, MAPK, gene expression |
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| 摘要 | Sugar transporters (Sts) play important roles in controlling carbohydrate transport and are responsible for mediating the movement of sugars into cells. Few studies have been conducted on expressions of Sts during insect embryonic development. In the present study, we investigated temporal expressions of St genes during the embryonic diapause process in Bombyx mori. We found that in HCl-treated developing eggs, high gene expressions of trehalose transporter 1 (Tret1) were detected during middle and later embryonic development. St4 and St3 gene expressions gradually increased during the early stages, reached a small peak on day 3, and large peaks were again detected on day 7. However, in diapause eggs, expression levels of the Tret1, St4, and St3 genes all remained at low levels. Differential temporal changes in expressions of the Tret1, St4, and St3 genes found between diapause and HCl-treated eggs were further confirmed using non-diapause eggs. Our results showed that non-diapause eggs exhibited similar changing patterns as those of HCl-treated eggs, thus clearly indicating potential correlations between expressions of these genes and embryonic development. In addition, high gene expressions of Tret1 were also detected when dechorionated eggs were incubated in medium. The addition of LY294002 (a specific phosphatidylinositol 3-kinase (PI3K) inhibitor) and U0126 (a mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) kinase (MEK) inhibitor) partially inhibited Tret1 gene expression in dechorionated eggs, but did not affect either ecdysteroid-phosphate phosphatase (EPPase) gene expression or ecdysteroid biosynthesis, clearly indicating that both PI3K and ERK are involved in increased gene expression of Tret1 that was in-dependent of ecdysteroid levels. To our knowledge, this is the first comprehensive report to demonstrate the transcriptional regulation of St genes during embryonic development, thus providing useful information for a clearer understanding of insect egg diapause mechanisms. |
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| 資料連結 | ![Full Text]() |
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| 計畫主持人 | 顧世紅 |
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| 計畫編號 | MOST 109-2313-B-178 -001 -MY3 |
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| 計畫年度 | 109 |
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| 計畫委託單位 | 國立自然科學博物館 |
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| 計畫名稱 | 一氧化氮參與昆蟲蛻皮激素合成之探討 |
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| 系統號 | NO000006858 |
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