學術資源整合系統-相關推薦
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| 作者 | Huang, Yu-Ling;Xiao, Jun-Xiang |
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| 出版日期 | 202306 |
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| 著作名稱 | Using herbarium collections to study fungal endophyte communities in leaves
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| 會議名稱 | SPNHC 2023 |
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| 會議地點 | SanFrancis |
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| 會議日期 | 20230528 |
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| 主辦單位 | SPNHC |
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| 國際性會議 | Y |
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| 主題 | 真菌;植物 |
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| 摘要 | Plant tissues contain cryptic diversity including pathogenic, epiphytic, endophytic fungi and other microorganisms. Fungal endophytes are fungi inhibiting within plant tissues without causing diseased symptoms. Ecological surveys of endophyte diversity require field sampling, fungal isolation, and identification. Fungal isolations from plant materials have to be finished in few days after sampling. Due to the geographic limitation, the number of acquirable plant specimens is limited, thus the depth and scale of fungal diversity and ecological studies might be restricted. With the advancement of DNA sequencing techniques, the high-throughput sequencing has become a mature technique and is widely used in the studies of fungal diversity in plants. Such studies usually use fresh plant materials and seldom use dried plant specimens for analyses. Herbarium plant specimens potentially preserve the fungal diversity. If fungal communities in fresh plants can be preserved in the dried plant specimens, we can know the variations of fungal communities from the past to the present and conduct the large-scale analyses in fungal ecology. Before using herbarium specimens to study the fungal ecology, three questions must be confirmed: (1) Are the endophyte communities different between fresh materials and dried specimens? (2) What are the differences of endophyte communities in the specimens deposited in different herbaria? (3) Do airborne fungi affect the fungal communities of herbarium specimens? This study analyzed the endophyte communities of the plant collections in the National Museum of Natural Science (TNM), the Herbarium of Biodiversity Research Center, Academia Sinica (HAST) and the Herbarium of Taiwan Forestry Research Institute (TAIF) in Taiwan, using Illumina Miseq paired-end sequencing method. Our results reveal that both fungal diversity and community structure are not different between fresh plants and dried specimens, and the fungal diversity of plant specimens deposited in the three herbaria are similar, while the community structures are significantly different. Additionally, few overlaps of fungal species between herbarium airborne fungi and plant specimens infers that the airborne fungi have limited effects on the fungal communities in plant specimens. Overall, herbarium collections are promising resources for studying the fungal diversity. |
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| 系統號 | NO000006766 |
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