學術資源整合系統-相關推薦
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| 作者 | Hu, W.H;Liu, S.F.; Liaw, S.I. |
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| 出版日期 | 2015 |
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| 著作名稱 | Long-term preconditioning of plantlets: a practical method for enhancing survival of pineapple (Ananas comosus (L.) Merr.) shoot tips cryopreserved using vitrification.
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| 刊名 | CryoLetters |
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| 卷 | 36 |
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| 期 | 4 |
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| 頁數 | 226-236 |
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| 被收錄索引 | SCI |
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| 摘要 | BACKGROUND: The purpose of this study was to develop an efficient cryopreservation protocol for pineapple (Ananas comosus (L.) Merr.) shoot tips. OBJECTIVE: The optimal state of pineapple plantlets was investigated by using sucrose preconditioning to enhance survival after cryostorage. METHODS: To achieve a suitable state of plantlets before cryopreservation, 0.2 M to 0.4 M sucrose concentrations combined with short- (0–7 days), medium- (15–30 days), and long-term (75–150 days) preconditioning periods were compared. RESULTS AND CONCLUSION: The highest survival (100%) was achieved using the following procedure: intact plantlets underwent long-term preconditioning with 0.2 M sucrose for 135 days, dissected shoot tips were treated with a loading solution containing 2.0 M glycerol 0.4 M sucrose for 60 min at 25°C and the shoot tips were dehydrated in PVS2 for 2 h at 0°C before being plunged in liquid nitrogen. Rewarming was conducted in a water-bath for 30 s at 40°C and PVS2 was replaced with a 1.2 M sucrose solution for 30 min at 25°C. The shoot tips were transferred on semisolid medium and left in the dark for 1 week, then in dim light for 3 weeks. |
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| 系統號 | NO000003906 |
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