學術資源整合系統-相關推薦
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| 作者 | Shi-Hong Gu;Chien-Hung Chen;Chia-Hao Chang;Pei-Ling Lin |
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| 出版日期 | 2023 |
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| 著作名稱 | Expression of tyrosine phosphatases in relation to PTTH-stimulated ecdysteroidogenesis in prothoracic glands of the silkworm, Bombyx mori
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| 刊名 | General and Comparative Endocrinology |
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| 卷 | 31 |
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| 頁數 | 114165 |
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| 被收錄索引 | SCI;SCI |
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| 主題 | 動物;動物 |
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| 關鍵字 | Bombyx mori; PTTH; Ecdysone; Tyrosine phosphatase; PTP1B; PTEN; Signaling. |
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| 摘要 | Protein tyrosine phosphorylation is a reversible, dynamic process regulated by the activities of tyrosine kinases and tyrosine phosphatases. Although the involvement of tyrosine kinases in the prothoracicotropic hormone (PTTH)-stimulated ecdysteroidogenesis in insect prothoracic glands (PGs) has been documented, few studies have been conducted on the involvement of tyrosine phosphatases in PTTH-stimulated PGs. In the present study, we examined the protein expressions of two important protein tyrosine phosphatases (protein tyrosine phosphatase 1B (PTP1B) and the phosphatase and tension homologue (PTEN)) in Bombyx mori PGs. Our immunoblotting analysis showed that Bombyx PGs contained the proteins of PTP1B and PTEN, with PTP1B protein undergoing development-specific changes during the last larval instar. Protein levels of PTP1B and PTEN were not affected by PTTH treatment. The mRNA expression levels of PTP1B and PTEN showed development-specific changes. Determination of PTP enzymatic activity showed that tyrosine phosphatase enzymatic activity was stimulated by PTTH treatment both in vitro and in vivo. Pretreatment with phenyl arsine oxide (PAO) and benzyl phosphonic acid (BPA), two chemical inhibitors of tyrosine phosphatase, reduced PTTH-stimulated enzymatic activity. Determination of ecdysteroid secretion showed that treatment with PAO and BPA did not affect basal ecdysteroid secretion, but greatly inhibited PTTH-stimulated ecdysteroid secretion, indicating that PTTH-stimulated tyrosine phosphatase activity is indeed involved in ecdysteroid secretion. PTTH-stimulated phosphorylation of the extracellular signal-regulated kinase (ERK) and 4E-binding protein (4E-BP) was partially inhibited by pretreatment with either PAO or BPA. From these results, we hypothesize that PTTH-regulated tyrosine phosphatase signaling may interfere with ERK and target of rapamycin (TOR) signaling pathways and is a necessary component for stimulation of ecdysteroid secretion. |
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| 資料連結 |  |
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| 計畫主持人 | 顧世紅 |
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| 計畫編號 | MOST 109-2313-B-178 -001 -MY3 |
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| 計畫年度 | 109 |
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| 計畫委託單位 | 國立自然科學博物館 |
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| 計畫名稱 | 一氧化氮參與昆蟲蛻皮激素合成之探討 |
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| 系統號 | NO000006857 |
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