學術資源整合系統-相關推薦
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| 作者 | Wei-Hsin Hu;Shu-Fen Tsai;Song-Iuan Liaw |
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| 出版日期 | 202112 |
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| 已接受 | 202201 |
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| 接受證明 |  |
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| 著作名稱 | CRYOPRESERVATION OF SWEET POTATO USING VITRIFICATION TECHNIQUE: EFFECTS OF THE VITRIFICATION PROCEDURE AND SUCROSE PRETREATMENTS
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| 刊名 | Propagation of Ornamental Plants |
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| 卷 | 21 |
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| 期 | 4 |
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| 頁數 | 123-130 |
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| 被收錄索引 | SCI |
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| 主題 | 植物 |
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| 關鍵字 | cryoprotection, physiological hardening, plant vitrification solution, ornamental |
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| 摘要 | An effective vitrification procedure was developed for sweet potato cryopreservation, which was applied to three accessions (Tainung Nos. 25, 64, and 66). Effects of 0.4 M sucrose pretreatment as well as durations of loading and dehydration with plant vitrification solution 2 (PVS2) on the survival of shoot tips were examined. In the optimal vitrification procedure, explant pretreatment by stepwise increases in sucrose concentrations up to 1.0 M was used to investigate recalcitrant accessions. Three days of 0.4 M sucrose pretreatment significantly improved the survival rates of shoot tips immersed in liquid nitrogen. The highest cryopreservation survival rates were obtained with 60-min osmoprotection and 75-min PVS2 dehydration. Survival rates of recalcitrant accessions could be improved with a suitable pretreatment concentration. The sucrose pretreatment concentration was optimized for the intact explants of each accession type (TN66: 0.4 M, TN64: 0.5 M, and TN25: 0.8 M). The highest survival rates of 86.6% (TN66), 76.6% (TN64), and 50.0% (TN25) were achieved using pretreatment for 3 days, loading for 60 min, and dehydration in PVS2 for 75 min. This vitrification procedure with high concentrations of sucrose pretreatment can improve the survival rates of shoot tips after cryopreservation. |
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| 系統號 | NO000006578 |
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