學術資源整合系統-相關推薦

 
作者Wei-Hsin Hu;Shu-Fen Tsai;Song-Iuan Liaw
出版日期202112
已接受202201
接受證明Full Text
著作名稱CRYOPRESERVATION OF SWEET POTATO USING VITRIFICATION TECHNIQUE: EFFECTS OF THE VITRIFICATION PROCEDURE AND SUCROSE PRETREATMENTS
刊名Propagation of Ornamental Plants
21
4
頁數123-130
被收錄索引SCI
主題植物
關鍵字cryoprotection, physiological hardening, plant vitrification solution, ornamental
摘要An effective vitrification procedure was developed for sweet potato cryopreservation, which was applied to three accessions (Tainung Nos. 25, 64, and 66). Effects of 0.4 M sucrose pretreatment as well as durations of loading and dehydration with plant vitrification solution 2 (PVS2) on the survival of shoot tips were examined. In the optimal vitrification procedure, explant pretreatment by stepwise increases in sucrose concentrations up to 1.0 M was used to investigate recalcitrant accessions. Three days of 0.4 M sucrose pretreatment significantly improved the survival rates of shoot tips immersed in liquid nitrogen. The highest cryopreservation survival rates were obtained with 60-min osmoprotection and 75-min PVS2 dehydration. Survival rates of recalcitrant accessions could be improved with a suitable pretreatment concentration. The sucrose pretreatment concentration was optimized for the intact explants of each accession type (TN66: 0.4 M, TN64: 0.5 M, and TN25: 0.8 M). The highest survival rates of 86.6% (TN66), 76.6% (TN64), and 50.0% (TN25) were achieved using pretreatment for 3 days, loading for 60 min, and dehydration in PVS2 for 75 min. This vitrification procedure with high concentrations of sucrose pretreatment can improve the survival rates of shoot tips after cryopreservation.
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系統號NO000006578

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