學術資源整合系統-相關推薦
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| 作者 | U’Ren, Jana M.;Massimo, N.;Fiddle, J.M.;Steen, C.;Arendt, K.;Huang, Yu-Ling;Miadlikowska, J.;Lefevre, E.;Ball, B.;Wong, V.L.;Monacell, J.;Carbone, I.;Lutzoni, F.;May, G.;Arnold, A.E. |
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| 出版日期 | 20130801 |
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| 著作名稱 | A culture-based and culture-free assessment of the geographic and temporal variation of boreal endophytic and endolichenic fungal communities.
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| 會議名稱 | MSA2013 |
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| 會議地點 | Austin, TX |
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| 會議日期 | 20130801 |
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| 主辦單位 | APS-MSA |
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| 國際性會議 | Y |
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| 主題 | 真菌 |
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| 摘要 | Although species-rich in all terrestrial communities, endophytic and endolichenic fungi reach their greatest phylogenetic diversity in boreal forests -- earth’s largest and most threatened forest biome. Previous studies have used single sampling events and culture-based methods to infer their spatial-, geographic-, and host affiliations. Here we pair culture-based and culture-free methods to examine endophytic and endolichenic communities in sets of 20 plant and lichen species sampled in multiple years at one boreal site, and in multiple temperate, temperate-montane, and boreal forest communities across the western hemisphere. In addition to revealing previously unexplored temporal variation in symbiotroph associations, detecting geographic structure among congeneric but allopatric hosts, and highlighting the complementarity of culture-based and culture-free methods, our application of 454 pyrosequencing shows how different tissue-storage and sequence analysis methods yield markedly different estimates of diversity. Further, we show that mycobiont sequences can provide controls for detecting pyrosequencing errors that would go undetected by the otherwise stringent quality control measures that are current in the field. Together these efforts reveal the relative contributions and biases of culture-based and culture-free methods in endophyte studies, and provide a first perspective on the symbionts of iconic hosts that are the foundation of boreal forest communities. |
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| 系統號 | NO000006469 |
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