學術資源整合系統-相關推薦
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| 作者 | Gu Shi-Hong et al |
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| 出版日期 | 202002 |
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| 著作名稱 | Reactive oxygen species-mediated bombyxin signaling in Bombyx mori
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| 刊名 | Insect Biochemistry and Molecular Biology |
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| 卷 | 117 |
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| 期 | 2 |
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| 頁數 | 103279 |
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| 被收錄索引 | SCI;SCI |
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| 主題 | 動物;動物 |
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| 關鍵字 | Bombyx mori; bombyxin; ROS; ecdysone; prothoracic glands; signal transduction; redox regulation. |
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| 摘要 | In the present study, we demonstrated that bombyxin, an insect insulin-like peptide, modulated ecdysteroidogenesis in Bombyx mori prothoracic glands (PGs) through redox signaling. Our results showed that bombyxin treatment resulted in a rapidly transient increase in intracellular reactive oxygen species (ROS) concentration, as measured using 2',7'-dichlorofluorescin diacetate (DCFDA), an oxidation-sensitive fluorescent probe. The antioxidant N-acetylcysteine (NAC) abolished the bombyxin-induced increase in fluorescence in Bombyx PGs. Furthermore, bombyxin-induced ROS production was inhibited by mitochondrial oxidative phosphorylation inhibitors (rotenone and antimycin A), indicating mitochondria-mediated ROS production. The stimulation of ROS production in response to bombyxin appears to undergo development-specific changes. We further investigated the action mechanism of bombyxin-stimulated ROS signaling. Results showed that in the presence of either NAC, rotenone, or antimycin A, bombyxin-stimulated phosphorylation of insulin receptor, Akt, and 4E-BP was blocked and bombyxin-stimulated ecdysteroidogenesis in PGs was greatly inhibited. From these results, we conclude that bombyxin-stimulated ROS signaling appears to be involved in bombyxin-stimulated ecdysteroidogenesis of PGs in B. mori by modulating the phosphorylation of insulin receptor, Akt, and 4E-BP. To our knowledge, this is the first demonstration of redox regulation in insulin signaling in an insect system. |
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| 計畫主持人 | 顧世紅 |
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| 計畫委託單位 | 科技部 |
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| 計畫名稱 | 活性氧與昆蟲蛻皮激素的分泌 |
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| 系統號 | NO000005781 |
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