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作者 Gu Shi-Hong et al
出版日期202002
著作名稱Reactive oxygen species-mediated bombyxin signaling in Bombyx mori
刊名Insect Biochemistry and Molecular Biology
117
2
頁數103279
被收錄索引SCI;SCI
主題動物;動物
關鍵字Bombyx mori; bombyxin; ROS; ecdysone; prothoracic glands; signal transduction; redox regulation.
摘要In the present study, we demonstrated that bombyxin, an insect insulin-like peptide, modulated ecdysteroidogenesis in Bombyx mori prothoracic glands (PGs) through redox signaling. Our results showed that bombyxin treatment resulted in a rapidly transient increase in intracellular reactive oxygen species (ROS) concentration, as measured using 2',7'-dichlorofluorescin diacetate (DCFDA), an oxidation-sensitive fluorescent probe. The antioxidant N-acetylcysteine (NAC) abolished the bombyxin-induced increase in fluorescence in Bombyx PGs. Furthermore, bombyxin-induced ROS production was inhibited by mitochondrial oxidative phosphorylation inhibitors (rotenone and antimycin A), indicating mitochondria-mediated ROS production. The stimulation of ROS production in response to bombyxin appears to undergo development-specific changes. We further investigated the action mechanism of bombyxin-stimulated ROS signaling. Results showed that in the presence of either NAC, rotenone, or antimycin A, bombyxin-stimulated phosphorylation of insulin receptor, Akt, and 4E-BP was blocked and bombyxin-stimulated ecdysteroidogenesis in PGs was greatly inhibited. From these results, we conclude that bombyxin-stimulated ROS signaling appears to be involved in bombyxin-stimulated ecdysteroidogenesis of PGs in B. mori by modulating the phosphorylation of insulin receptor, Akt, and 4E-BP. To our knowledge, this is the first demonstration of redox regulation in insulin signaling in an insect system.
計畫主持人顧世紅
計畫委託單位科技部
計畫名稱活性氧與昆蟲蛻皮激素的分泌
系統號NO000005781

Aug 16 2018 10:46:44
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