學術資源整合系統-相關推薦

 
作者Gu Shi-Hong et al
出版日期202002
著作名稱Expression of protein kinase C in relation to the embryonic diapause process in the silkworm, Bombyx mori
刊名Journal of Insect Physiology
121
104010
頁數1-8
被收錄索引SCI;SCI
主題動物;動物
關鍵字Bombyx mori PKC Phosphorylation Embryonic diapause Signaling
摘要In the present study, we investigated the expression of protein kinase C (PKC) signaling during the embryonic diapause process of Bombyx mori. PKC activity, determined using an antibody to phosphorylated substrates of PKC, was found to be significantly higher in developing eggs as compared to that of diapause eggs. In eggs whose diapause initiation was prevented by HCl, non-diapause eggs, and eggs in which diapause had been terminated by chilling of diapausing eggs at 5 °C for 70 days and then were transferred to 25 °C, PKC-dependent phosphorylation levels of multiple proteins showed dramatic stage-dependent increases compared to those of diapause eggs. Higher protein levels of PKC were also detected in developing eggs as compared to those of diapause eggs. Determination of PKC enzymatic activity during the middle embryonic stage showed higher PKC activity in developing eggs compared to diapause eggs, thus further confirming differential regulation of PKC signaling during the embryonic diapause process. Examination of temporal changes in mRNA expression levels of classical PKC (cPKC) and atypical PKC (aPKC) showed no difference between diapause and HCl-treated eggs. These results demonstrated that differential expressions of PKC signaling between diapause and developing eggs are related to the embryonic diapause process of B. mori.
計畫主持人顧世紅
計畫年度2019
計畫委託單位科技部
計畫名稱活性氧與昆蟲蛻皮激素的分泌
系統號NO000005779

Aug 16 2018 10:46:44
nmns/nmnsweb_2nd_target(0)