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作者Gu, S.H.;Chen, C.H.;Lin, P.L.;Hsieh,H.Y.
出版日期2019
著作名稱Role of protein phosphatase 2A in PTTH-stimulated prothoracic glands of the silkworm, Bombyx mori
刊名General and Comparative Endocrinology
274
頁數97-105
被收錄索引SCI;SCI
主題動物;動物
關鍵字Bombyx mori; PTTH; Ecdysone; PP2A; Dephosphorylation; Protein phosphatases.
摘要In the present study, the roles of a major serine/threonine protein phosphatase 2A (PP2A) in prothoracicotropic hormone (PTTH)-stimulated prothoracic glands (PGs) of Bombyx mori were evaluated. Immunoblotting analysis showed that Bombyx PGs contained a structural A subunit (A), a regulatory B subunit (B), and a catalytic C subunit (C), with each subunit undergoing development-specific changes. The protein levels of each subunit were not affected by PTTH treatment. However, the highly conserved tyrosine dephosphorylation of PP2A C subunit (PP2AC), which appears to be related to activity, was increased by PTTH treatment in a time-dependent manner. We further demonstrated that phospholipase C (PLC), Ca2+, and reactive oxygen species (ROS) are upstream signaling for the PTTH-stimulated dephosphorylation of PP2AC. The determination of PP2A enzymatic activity showed that PP2A enzymatic activity was stimulated by PTTH treatment both in vitro and in vivo. Okadaic acid (OA), a specific PP2A inhibitor, prevented the PTTH-stimulated dephosphorylation of PP2AC and reduced both basal and PTTH-stimulated PP2A enzymatic activity. The determination of ecdysteroid secretion showed that treatment with OA did not affect basal ecdysteroid secretion but did significantly inhibit PTTH-stimulated ecdysteroid secretion, indicating that PTTH-stimulated PP2A activity is involved in ecdysteroidogenesis. Treatment with OA stimulated the basal phosphorylation of the extracellular signal-regulated kinase (ERK) and 4E-binding protein (4E-BP) without affecting PTTH-stimulated ERK and 4E-BP phosphorylation. From these results, we hypothesize that PTTH-regulated PP2A signaling is a necessary component for the stimulation of ecdysteroidogenesis, potentially by mediating the link between ERK and TOR signaling pathways.
計畫主持人顧世紅
計畫編號MOST 107-2313-B178-001
計畫年度2018
計畫委託單位科技部
計畫名稱protein phosphatases 與昆蟲蛻皮激素的分泌
系統號NO000005113

Aug 16 2018 10:46:44
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