學術資源整合系統-相關推薦
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| 作者 | Gu, S.H.;Chen, C.H.;Lin, P.L.;Hsieh,H.Y. |
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| 出版日期 | 2019 |
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| 著作名稱 | Role of protein phosphatase 2A in PTTH-stimulated prothoracic glands of the silkworm, Bombyx mori
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| 刊名 | General and Comparative Endocrinology |
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| 卷 | 274 |
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| 頁數 | 97-105 |
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| 被收錄索引 | SCI;SCI |
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| 主題 | 動物;動物 |
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| 關鍵字 | Bombyx mori; PTTH; Ecdysone; PP2A; Dephosphorylation; Protein phosphatases. |
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| 摘要 | In the present study, the roles of a major serine/threonine protein phosphatase 2A (PP2A) in prothoracicotropic hormone (PTTH)-stimulated prothoracic glands (PGs) of Bombyx mori were evaluated. Immunoblotting analysis showed that Bombyx PGs contained a structural A subunit (A), a regulatory B subunit (B), and a catalytic C subunit (C), with each subunit undergoing development-specific changes. The protein levels of each subunit were not affected by PTTH treatment. However, the highly conserved tyrosine dephosphorylation of PP2A C subunit (PP2AC), which appears to be related to activity, was increased by PTTH treatment in a time-dependent manner. We further demonstrated that phospholipase C (PLC), Ca2+, and reactive oxygen species (ROS) are upstream signaling for the PTTH-stimulated dephosphorylation of PP2AC. The determination of PP2A enzymatic activity showed that PP2A enzymatic activity was stimulated by PTTH treatment both in vitro and in vivo. Okadaic acid (OA), a specific PP2A inhibitor, prevented the PTTH-stimulated dephosphorylation of PP2AC and reduced both basal and PTTH-stimulated PP2A enzymatic activity. The determination of ecdysteroid secretion showed that treatment with OA did not affect basal ecdysteroid secretion but did significantly inhibit PTTH-stimulated ecdysteroid secretion, indicating that PTTH-stimulated PP2A activity is involved in ecdysteroidogenesis. Treatment with OA stimulated the basal phosphorylation of the extracellular signal-regulated kinase (ERK) and 4E-binding protein (4E-BP) without affecting PTTH-stimulated ERK and 4E-BP phosphorylation. From these results, we hypothesize that PTTH-regulated PP2A signaling is a necessary component for the stimulation of ecdysteroidogenesis, potentially by mediating the link between ERK and TOR signaling pathways. |
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| 計畫主持人 | 顧世紅 |
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| 計畫編號 | MOST 107-2313-B178-001 |
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| 計畫年度 | 2018 |
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| 計畫委託單位 | 科技部 |
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| 計畫名稱 | protein phosphatases 與昆蟲蛻皮激素的分泌 |
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| 系統號 | NO000005113 |
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