學術資源整合系統-相關推薦

 
作者Huang, Yu-Ling;Zimmerman, Naupaka B.;Arnold, A. Elizabeth
出版日期201805
已接受201805
著作名稱Observations on the early establishment of foliar endophytic fungi in leaf discs and living leaves of a model woody angiosperm, Populus trichocarpa (Salicaceae).
刊名Journal of Fungi
4
2
頁數58
主題真菌
關鍵字Cladosporium; Penicillium; Populus; Trichoderma; colonization; microscopy; stomata
摘要Fungal endophytes are diverse and widespread symbionts that occur in the living tissues of all lineages of plants without causing evidence of disease. Culture-based and culture-free studies indicate that they often are abundant in the leaves of woody angiosperms, but only a few studies have visualized endophytic fungi in leaf tissues, and the process through which most endophytes colonize leaves has not been studied thoroughly. We inoculated leaf discs and the living leaves of a model woody angiosperm, Populus trichocarpa, which has endophytes that represent three distantly-related genera (Cladosporium, Penicillium, and Trichoderma). We used scanning electron microscopy and light microscopy to evaluate the timeline and processes by which they colonize leaf tissue. Under laboratory conditions with high humidity, conidia germinated on leaf discs to yield hyphae that grew epiphytically and incidentally entered stomata, but did not grow in a directed fashion toward stomatal openings. No cuticular penetration was observed. The endophytes readily colonized the interiors of leaf discs that were detached from living leaves, and could be visualized within discs with light microscopy. Although they were difficult to visualize within the interior of living leaves following in vivo inoculations, standard methods for isolating foliar endophytes confirmed their presence.
全文Full Text
資料連結Full Text
DOIhttps://doi.org/10.3390/jof4020058
系統號NO000004466

Aug 16 2018 10:46:44
nmns/nmnsweb_2nd_target(0)