學術資源整合系統-相關推薦

 
作者Chen, Ying-Chun;Huang, Yang-Jung;Wang, Chiu-Mei;Chiu, Chin-An;Lin, Huey-Ling;Lee, Pei-Fang;Cheng, Ya-Ming;Chang, Chen
出版日期2017
著作名稱Re-emergence of Lilium callosum Sieb.et Zucc. in Taiwan after a fre allows propagationand renews the possibility of conservation
刊名Botanical Studies
58
1
頁數47-56
摘要Background: Lilium callosum is native to Taiwan, but little is known about it since it has been considered extinct since 1915. After the rediscovery of this rare species after a fre in 2011 in Tunghsiao Township, intensive work has been conducted to count the number in the wild population, to develop a conservation strategy, and to understand its reproductive characteristics and even economic potential.
Results: To conserve the germplasm of this population, three scales from a wild L. callosum plant were collected to establish a mass propagation system. Flowers from two regenerated plants were crossed by hand-pollination, the ovules were rescued and cultured in vitro, and 10 ofspring were obtained. The karyotype was determined to be 2n = 2x = 24 = 2m 2m(sat) 2sm 8st 10t. The phylogenetic analysis using ITS sequences revealed that the sample
of L. callosum from Taiwan was not grouped with the other accessions of L. callosum from other regions. The native habitat is classifed as grass-dominated vegetation at the early successional stage and a subtropical monsoon-type climate. To clarify the causes of population scarcity in the native environment, reproductive characteristics of regener-
ated plants were investigated.

Conclusions: Based on the information from this study, it is possible that factors intrinsic to L. callosum could combine to limit pollination and seed formation. The L. callosum pollen only germinated at a temperature that was higher than the native environment, the plants are self-incompatibile, there was a and scarce population, scattered fowering time and dichogamy. Through the culture of these wild harvested parts, the diversity of the germplasm has been broadened and is now available to preserve this rare and valuable species for the future.
系統號NO000004402

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